This study was devised to observe the effects of water extracts of the Trichosanthes semen and Sinapsis semen against the growth of some cancer cells in vitro and in vivo. There species of cancer cells, murine lymphoid leukemic cells, L1210, murine lymphocytic cells, P388, and murine ascitic Sarcoma 180 cells, were used for the experiment.
The effects of incubation time and concentration of the extracts in the culture medium against the growth of L1210 and P388 cells in vitro were determined. And survival time and results obtained are summarized as follows;
1. The doubling times of the L1210, P388 and Sarcoma 180 cells in culture medium without adding any extracts were 11, 11, and 26 hours, respectively.
2. The growth rates of the L1210 and P388 cells in the culture medium contained the extract of Trichosanthes semen(0.1 ml/5 ml) were 27.3% and 43.6%, respectively, and ED50 values of the both cells were belong to below 0.1 ml of the extract.
3. The growth rates of the L1210 and P388 cells in Sinapsis semen extract were 63.2% to 32.1% and 68.8% to 46.6%, respectively, and ED50 values of the both cells were in the range of 0.1ml to 0.2ml of the extract.
4. The inhibitoy effects of Trichosanthes semen and Sinapsis semen extracts on the growth rate of L1210 and P388 cells in vitro were increased, compared with the control group, by the extention of the incubation time and the increase of concentration of each extract in the culture medium.
5. The numbers of Sarcoma 180 cell ascite of Swiss mice were slightly decreased, compared with control group, by the administration of the both extracts.
6. The survival times of the Swiss mice inoculated with Sarcoma 180 cells were not affected by the administration of the both extracts.
According to the above results, it could be said that the water extracts of the Trichosanthes semen and Sinapsis semen were indicated the inhibitory effects on the growth of the L1210 and p388 cells in vitro and in vivo, but the survival time of Swiss mice inoculated with Sarcoma 180 cells could not extended by administration of the both extracts.
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